Our laboratory uses different optical methods, and we very often obtain really beautiful images which become later hidden behind traces or histograms. Here we would like to share some of them with visitors of our site.
A stack of images from a hippocampal astrocyte filled with BDA-10000 via a patch pipette and imaged with Olympus FV1000D system.
(by Ms Pei-Yu Shih)
Left:
A CA1 pyramidal neuron filled with FM-4-64 via a patch pipette and imaging with Olympus FV1000 system equipped with detectors for second harmonic generation (SHG) imaging.
Two-photon fluorescence image and SHG image were obtained simultaneously.
Right:
Monitoring voltage changes during backpropagating action potential
with point-scan SHG imaging. Action potentials could be recorded from soma (a) and a spine on basal dendrite(b).
(by Dr Sylvain Rama)
Two-photon imaging of a CA1 pyramidal neuron filled with Alexa 594 and Fluo4 via a patch pipette. Calcium transients during back propagating action potential (bAP) were obtained with line-scan calcium imagings from apical blique dendritic spins (s1 & s2) and shaft (de).
